An important biochemical reaction that is used to do work

An important biochemical reaction that is used to do work (on muscle or in transport of chemical species or in driving biosynthesis) involves AdenosineTriPhosphate (ATP) and AdenosineDiPhosphate (ADP) and can be written as:ATP H2O ==> ADP Pi– H ?Go = 9.2 kJ/molwhere Pi– is inorganic phosphate. In biological systems this reaction is to an excellent appoximation carried out at constant pressure (1 atm) and constant temperature.Is this reaction spontaneous when carried out with all reactants and products present at standard state thermodynamic conditions?Calculate ?G (in J) for the ATP–ADP reaction at 25 °C and the typical physiological conditions:pH = 6.5[ATP] = 0.00001 M[ADP] = 0.0004 M[Pi–] = 0.00025 M