Part I: Calculate the energy (in Joules) of a photon of light whose wavelength is 650nm (show wall work circle final answer!!)Part II:The formula for determining how many milliliters of a stock of 10ppm solution you’ll need to make each of your calibration curve solutions (2 4 6 8 ppm) is provided below. Using this formula along with information in your lab manual procedure calculate the volume of stock solution required to make each of your calibration curve solutions.I’ve provided an attachment of the worksheet with the last question on the second page the question number is 9.Name ____________________________________CHM 151 Pre-lab Preparation WorksheetDr. Anthony Warren Ph.D.Pre-labwrite-upwascompletedas requiredPropersplashgogglesavailableas requiredSpectrophotometric Determination of Concentration1) (2) What pages in your lab manual covers this experiment? page _______ through page _______2) (2) Which of the following best expresses the primary objective of the lab?a.b.c.d.Determining the concentration (ppm) of an unknown solution of red dye.Determining the concentration (ppm) of an unknown solution of yellow dye.Determining the concentration (ppm) of a known solution of red dye.Calculating the density of a saline solution containing green dye.3) (2) Whose law is utilized in this lab with regards to solution concentration and absorbance?a.b.c.d.Avocado’sBohr’sBeer’sMendeleev’s4) (3) Calculate the energy (in Joules) of a photon of light whose wavelength is 650 nm (show allwork circle final answer!!!!).5) (2) If you were determining the concentration of an unknown solution of blue dye over what rangeof wavelengths would you calibrate the spectrophotometer?__________ nm to __________ nm1 6) (2) Write the general equation for a straight line (slope-intercept form).7) (2) What is the approximate wavelength range of visible light in nanometers?__________ nm to __________ nm8) (2) Which of the following is used to hold your sample for measurement in the spectrophotometer?a.b.c.d.test tubemicrocapillary tubegraduated cylindercuvette9) (8) The formula for determining how many milliliters of a stock 10 ppm solution you’ll need tomake each of your calibration curve solutions (2 4 6 and 8 ppm) is provided below. Using thisformula along with information in your lab manual procedure calculate the volume of stocksolution required to make each of your calibration curve solutions.V1 = (C2/C1)*V2where V1 = the volume (mL) of stock solution neededC2 = the desired concentration of you diluted solution(i.e. a calibration curve solution)C1 = the concentration (ppm) of your stock solutionV2 = the desired volume (mL) of the diluted solution2
