Western Blot /Gel Electrophoresis/Isolate Protein


Please complete the following test:

-Western blot  for  G6PD deficiency

-Gel electrophoresis

-Isolate Protein

USE THE FOLLOWING SEQUENCE :

Forward Primer: 5’- AACCGCATCATCGTGGAGAAG-3′

Reverse Primer: 5’-AGCCGGTCAGAGCTCTGCAGG-3′

Restriction enzyme- Glam11

5’-AACGTT -3′

3’-TTGCAA-5′

5’-ACCGATCGACGTAA | ACGTTCGCAGATCTA | ACGTTCGCAGT-3′

3’-TGGCTAGCTGCATTTGCA | AGCGTCTAGATTGCA | AGCGTCA-5′

I NEED THIS DONE IN 6 (SIX ) HOURS

CLEAR PICTURES AND INFORMATION NEEDED