Please complete the following test:
-Western blot for G6PD deficiency
-Gel electrophoresis
-Isolate Protein
USE THE FOLLOWING SEQUENCE :
Forward Primer: 5’- AACCGCATCATCGTGGAGAAG-3′
Reverse Primer: 5’-AGCCGGTCAGAGCTCTGCAGG-3′
Restriction enzyme- Glam11
5’-AACGTT -3′
3’-TTGCAA-5′
5’-ACCGATCGACGTAA | ACGTTCGCAGATCTA | ACGTTCGCAGT-3′
3’-TGGCTAGCTGCATTTGCA | AGCGTCTAGATTGCA | AGCGTCA-5′
I NEED THIS DONE IN 6 (SIX ) HOURS
CLEAR PICTURES AND INFORMATION NEEDED